A Versatile Metagenome Purification Method to Identify Uncultivable Bacteria by Denaturing Gradient Gel Electrophoresis (DGGE) from Sediments and Soils
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چکیده
منابع مشابه
Detecting the Effects of Fungicides on Soil Bacteria Populations using Denaturing Gradient Gel Electrophoresis (DGGE)
Rationale Fungicide applications are a necessary practice to discourage disease development in most high quality turfgrass areas. Little is known, however, about the effects that fungicides have on resident microbial community of the soils supporting turfgrass sites. Soil bacteria are responsible for important processes including pesticide degradation, nutrient cycling, and organic matter break...
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Although freshwater ecosystems support a great diversity of life, knowledge of its total diversity is far to be complete, particularly among microbes (Dudgeon et al., 2006). Aquatic microorganisms are pivotal in several biogeochemical processes, playing a key role in the recycling of organic material, or contributing to energy transfer in food webs, since they constitute food sources to micro-f...
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16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.
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Many phyla of marine invertebrates are difficult to identify using conventional morphological taxonomy. Larvae of a wider set of phyla are also difficult to identify as a result of conservation of morphology between species or because morphological characters are destroyed during sampling and preservation. DNA sequence analysis has the potential for identification of marine organisms to the spe...
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ژورنال
عنوان ژورنال: Journal of Bacteriology & Parasitology
سال: 2012
ISSN: 2155-9597
DOI: 10.4172/2155-9597.1000147